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The document evaluates the environmental sustainability of first-generation biofuels produced in the Basque Country using an innovative methodology that integrates the SIMSNIC agronomic model with life cycle analysis. The study focuses on bioethanol from wheat and biodiesel from rapeseed in the La Llanada Alavesa area. It finds that while biofuels are more energy efficient than fossil fuels, meeting the EU's mandatory 35% greenhouse gas (GHG) reduction target is unlikely under current mineral fertilisation conditions, particularly in nitrate-vulnerable areas. Organic fertilisation increases the likelihood of meeting GHG targets but exacerbates water contamination, soil acidification, and land-use competition. The authors conclude that the agricultural stage, specifically nitrogen fertilisation and climatology, is the primary driver of the carbon footprint due to nitrous oxide emissions.