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The document outlines how to apply a human rights-based approach (HRBA) to the Kunming-Montreal Global Biodiversity Framework (GBF), emphasizing the distinction between duty bearers and rights holders. It provides detailed guidance on identifying vulnerable groups—including Indigenous Peoples, women, youth, and environmental human rights defenders—and integrating principles of equity, participation, and access to justice into biodiversity planning, financing, and monitoring. The guide specifically maps the 23 GBF Targets to human rights actions, advocating for the recognition of traditional territories, the implementation of Free, Prior and Informed Consent (FPIC), and the adoption of gender-responsive policies to ensure that biodiversity conservation does not lead to human rights violations.